Incubation:Article Title: Kiaa0101 serves as a prognostic marker and promotes invasion by regulating p38/snail1 pathway in glioma
Article Snippet: .. The contents were then incubated with secondary antibody (Lincoln, NE, USA; 1:10,000) in darkness for 1 h. The experiment was performed in triplicate. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Supplier Catalog number Dilution GAPDH Proteintech 60004-1-Ig 1:5,000 Kiaa0101 Sigma WH0009768M1 1:1,000 MMP2 Proteintech 10373-2-AP 1:1,000 Snail1 Proteintech 13099-1-AP 1:1,000 Vimentin Proteintech 10366-1-AP 1:1,000 E-cadherin Abcam ab15148 1:500 P38 Cell signaling technology 9212S 1:1,000 p-p38 (Thr180/Tyr182) Cell signaling technology 4511S 1:1,000 ERK Cell signaling technology 4695S 1:1,000 (p44/42) p-ERK1/2 Cell signaling technology 9101S 1:1,000 JNK Abcam ab179461 1:1,000 P-SAPK/JNK (Tyr185) Sigma SAB4504450 1:1,000 Open in a separate window caption a8 Primary antibodies used in this study Construction and transfection with shRNA and plasmid Oligonucleotides encoding shRNA targeted Kiaa0101 and a scrambled shRNA were purchased from Genechem Co., Ltd (Shanghai, China). ..
Article Title: Kiaa0101 serves as a prognostic marker and promotes invasion by regulating p38/snail1 pathway in glioma
Article Snippet: .. The contents were then incubated with secondary antibody (Lincoln, NE, USA; 1:10,000) in darkness for 1 h. The experiment was performed in triplicate. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Supplier Catalog number Dilution GAPDH Proteintech 60004-1-Ig 1:5,000 Kiaa0101 Sigma WH0009768M1 1:1,000 MMP2 Proteintech 10373-2-AP 1:1,000 Snail1 Proteintech 13099-1-AP 1:1,000 Vimentin Proteintech 10366-1-AP 1:1,000 E-cadherin Abcam ab15148 1:500 P38 Cell signaling technology 9212S 1:1,000 p-p38 (Thr180/Tyr182) Cell signaling technology 4511S 1:1,000 ERK Cell signaling technology 4695S 1:1,000 (p44/42) p-ERK1/2 Cell signaling technology 9101S 1:1,000 JNK Abcam ab179461 1:1,000 P-SAPK/JNK (Tyr185) Sigma SAB4504450 1:1,000 Open in a separate window caption a8 Primary antibodies used in this study .. Oligonucleotides encoding shRNA targeted Kiaa0101 and a scrambled shRNA were purchased from Genechem Co., Ltd (Shanghai, China).
Transfection:Article Title: Kiaa0101 serves as a prognostic marker and promotes invasion by regulating p38/snail1 pathway in glioma
Article Snippet: .. The contents were then incubated with secondary antibody (Lincoln, NE, USA; 1:10,000) in darkness for 1 h. The experiment was performed in triplicate. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Supplier Catalog number Dilution GAPDH Proteintech 60004-1-Ig 1:5,000 Kiaa0101 Sigma WH0009768M1 1:1,000 MMP2 Proteintech 10373-2-AP 1:1,000 Snail1 Proteintech 13099-1-AP 1:1,000 Vimentin Proteintech 10366-1-AP 1:1,000 E-cadherin Abcam ab15148 1:500 P38 Cell signaling technology 9212S 1:1,000 p-p38 (Thr180/Tyr182) Cell signaling technology 4511S 1:1,000 ERK Cell signaling technology 4695S 1:1,000 (p44/42) p-ERK1/2 Cell signaling technology 9101S 1:1,000 JNK Abcam ab179461 1:1,000 P-SAPK/JNK (Tyr185) Sigma SAB4504450 1:1,000 Open in a separate window caption a8 Primary antibodies used in this study Construction and transfection with shRNA and plasmid Oligonucleotides encoding shRNA targeted Kiaa0101 and a scrambled shRNA were purchased from Genechem Co., Ltd (Shanghai, China). ..
shRNA:Article Title: Kiaa0101 serves as a prognostic marker and promotes invasion by regulating p38/snail1 pathway in glioma
Article Snippet: .. The contents were then incubated with secondary antibody (Lincoln, NE, USA; 1:10,000) in darkness for 1 h. The experiment was performed in triplicate. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Supplier Catalog number Dilution GAPDH Proteintech 60004-1-Ig 1:5,000 Kiaa0101 Sigma WH0009768M1 1:1,000 MMP2 Proteintech 10373-2-AP 1:1,000 Snail1 Proteintech 13099-1-AP 1:1,000 Vimentin Proteintech 10366-1-AP 1:1,000 E-cadherin Abcam ab15148 1:500 P38 Cell signaling technology 9212S 1:1,000 p-p38 (Thr180/Tyr182) Cell signaling technology 4511S 1:1,000 ERK Cell signaling technology 4695S 1:1,000 (p44/42) p-ERK1/2 Cell signaling technology 9101S 1:1,000 JNK Abcam ab179461 1:1,000 P-SAPK/JNK (Tyr185) Sigma SAB4504450 1:1,000 Open in a separate window caption a8 Primary antibodies used in this study Construction and transfection with shRNA and plasmid Oligonucleotides encoding shRNA targeted Kiaa0101 and a scrambled shRNA were purchased from Genechem Co., Ltd (Shanghai, China). ..
Plasmid Preparation:Article Title: Kiaa0101 serves as a prognostic marker and promotes invasion by regulating p38/snail1 pathway in glioma
Article Snippet: .. The contents were then incubated with secondary antibody (Lincoln, NE, USA; 1:10,000) in darkness for 1 h. The experiment was performed in triplicate. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody Supplier Catalog number Dilution GAPDH Proteintech 60004-1-Ig 1:5,000 Kiaa0101 Sigma WH0009768M1 1:1,000 MMP2 Proteintech 10373-2-AP 1:1,000 Snail1 Proteintech 13099-1-AP 1:1,000 Vimentin Proteintech 10366-1-AP 1:1,000 E-cadherin Abcam ab15148 1:500 P38 Cell signaling technology 9212S 1:1,000 p-p38 (Thr180/Tyr182) Cell signaling technology 4511S 1:1,000 ERK Cell signaling technology 4695S 1:1,000 (p44/42) p-ERK1/2 Cell signaling technology 9101S 1:1,000 JNK Abcam ab179461 1:1,000 P-SAPK/JNK (Tyr185) Sigma SAB4504450 1:1,000 Open in a separate window caption a8 Primary antibodies used in this study Construction and transfection with shRNA and plasmid Oligonucleotides encoding shRNA targeted Kiaa0101 and a scrambled shRNA were purchased from Genechem Co., Ltd (Shanghai, China). ..
|
Proteintech is a verified supplier 